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014850 whole human genome microarray 4 × 44k g4112f  (Agilent technologies)


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    Structured Review

    Agilent technologies 014850 whole human genome microarray 4 × 44k g4112f
    014850 Whole Human Genome Microarray 4 × 44k G4112f, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+44k+microarray/pm39199011-30-29-32
    Average 90 stars, based on 1 article reviews
    014850 whole human genome microarray 4 × 44k g4112f - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: M6A-mediated molecular patterns and tumor microenvironment infiltration characterization in nasopharyngeal carcinoma
    Article Snippet: The mRNA expression data of the GSE102349 dataset (Submission date: Aug 08, 2017, Last update date: May 15, 2019, data retrieval: Jan 08, 2022) was generated using Illumina HiSeq 2000, this dataset includes mRNA expression data and clinical information from 113 NPC patients, and 88 cases with progression-free survival (PFS) involved in this study. .. And the mRNA expression data of the GSE53819 dataset (Submission date: Jan 04, 2014, Last update date: Aug 01, 2019, data retrieval: Jan 08, 2022) was generated using Agilent -014,850 Whole Human Genome Microarray 4 × 44K G4112F, and this dataset contained 18 NPC primary tumor tissues and 18 non-cancerous nasopharyngeal tissues. ..

    Generated:

    Article Title: M6A-mediated molecular patterns and tumor microenvironment infiltration characterization in nasopharyngeal carcinoma
    Article Snippet: The mRNA expression data of the GSE102349 dataset (Submission date: Aug 08, 2017, Last update date: May 15, 2019, data retrieval: Jan 08, 2022) was generated using Illumina HiSeq 2000, this dataset includes mRNA expression data and clinical information from 113 NPC patients, and 88 cases with progression-free survival (PFS) involved in this study. .. And the mRNA expression data of the GSE53819 dataset (Submission date: Jan 04, 2014, Last update date: Aug 01, 2019, data retrieval: Jan 08, 2022) was generated using Agilent -014,850 Whole Human Genome Microarray 4 × 44K G4112F, and this dataset contained 18 NPC primary tumor tissues and 18 non-cancerous nasopharyngeal tissues. ..

    Microarray:

    Article Title: M6A-mediated molecular patterns and tumor microenvironment infiltration characterization in nasopharyngeal carcinoma
    Article Snippet: The mRNA expression data of the GSE102349 dataset (Submission date: Aug 08, 2017, Last update date: May 15, 2019, data retrieval: Jan 08, 2022) was generated using Illumina HiSeq 2000, this dataset includes mRNA expression data and clinical information from 113 NPC patients, and 88 cases with progression-free survival (PFS) involved in this study. .. And the mRNA expression data of the GSE53819 dataset (Submission date: Jan 04, 2014, Last update date: Aug 01, 2019, data retrieval: Jan 08, 2022) was generated using Agilent -014,850 Whole Human Genome Microarray 4 × 44K G4112F, and this dataset contained 18 NPC primary tumor tissues and 18 non-cancerous nasopharyngeal tissues. ..

    Article Title: Method for reconstructing immune function using pluripotent stem cells
    Article Snippet: Respective total RNAs were extracted from human ES cells, T-iPS cells, redifferentiated T cells, peripheral blood T cells, and peripheral blood NK cells using an RNeasy® Micro Kit (manufactured by Qiagen). .. Fluorescently labeled complementary RNAs of each of the total RNAs and Whole Human Genome Microarray 4×44K (G4112F, manufactured by Agilent Technologies) or SurePrint G3 Human Gene Expression 8×60K (G4851A, manufactured by Agilent Technologies) were hybridized in a one-color protocol. .. The resulting signal data were analyzed using a GeneSpring GX software (manufactured by Agilent Technologies).

    Article Title: Method for reconstructing immune function using pluripotent stem cells
    Article Snippet: PCR reactions were carried out using ExTaq HS (manufactured by Takara) for 30 cycles for a housekeeping gene (GAPDH or ACTB) and for 35 cycles for all pluripotent genes and T-cell-related genes. .. Then, fluorescently labeled complementary RNAs from each of the total RNAs and Whole Human Genome Microarray 4×44K (G4112F, manufactured by Agilent Technologies) were hybridized in a one-color protocol (outsourcing to Takara Bio Inc.). .. Subsequently, the arrays were scanned and their spot images were detected on an Agilent Feature Extraction instrument (manufactured by Agilent Technologies).

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay
    Article Snippet: 200 ng total RNA was transcribed into cDNA with an oligo-dT primer, followed by transcription to cRNA labeled with cyanine 3-CTP (Quick-Amp Labeling Kit, One-color, Agilent). cRNA purification was performed with the RNeasy Mini Kit (Qiagen) and dye incorporation and cRNA yields were measured with the NanoDrop-1000 spectrophotometer (Thermofisher). .. Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a hybridization oven (Agilent) at 65°C for 17 h. After hybridization, DNA microarrays were washed, and slides were immediately scanned with the Microarray Scanner (G2505 B, Agilent) as recommended by Agilent.

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.

    Labeling:

    Article Title: Method for reconstructing immune function using pluripotent stem cells
    Article Snippet: Respective total RNAs were extracted from human ES cells, T-iPS cells, redifferentiated T cells, peripheral blood T cells, and peripheral blood NK cells using an RNeasy® Micro Kit (manufactured by Qiagen). .. Fluorescently labeled complementary RNAs of each of the total RNAs and Whole Human Genome Microarray 4×44K (G4112F, manufactured by Agilent Technologies) or SurePrint G3 Human Gene Expression 8×60K (G4851A, manufactured by Agilent Technologies) were hybridized in a one-color protocol. .. The resulting signal data were analyzed using a GeneSpring GX software (manufactured by Agilent Technologies).

    Article Title: Method for reconstructing immune function using pluripotent stem cells
    Article Snippet: PCR reactions were carried out using ExTaq HS (manufactured by Takara) for 30 cycles for a housekeeping gene (GAPDH or ACTB) and for 35 cycles for all pluripotent genes and T-cell-related genes. .. Then, fluorescently labeled complementary RNAs from each of the total RNAs and Whole Human Genome Microarray 4×44K (G4112F, manufactured by Agilent Technologies) were hybridized in a one-color protocol (outsourcing to Takara Bio Inc.). .. Subsequently, the arrays were scanned and their spot images were detected on an Agilent Feature Extraction instrument (manufactured by Agilent Technologies).

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay
    Article Snippet: 200 ng total RNA was transcribed into cDNA with an oligo-dT primer, followed by transcription to cRNA labeled with cyanine 3-CTP (Quick-Amp Labeling Kit, One-color, Agilent). cRNA purification was performed with the RNeasy Mini Kit (Qiagen) and dye incorporation and cRNA yields were measured with the NanoDrop-1000 spectrophotometer (Thermofisher). .. Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent). .. For hybridization, DNA microarrays were placed into a hybridization oven (Agilent) at 65°C for 17 h. After hybridization, DNA microarrays were washed, and slides were immediately scanned with the Microarray Scanner (G2505 B, Agilent) as recommended by Agilent.

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.

    Gene Expression:

    Article Title: Method for reconstructing immune function using pluripotent stem cells
    Article Snippet: Respective total RNAs were extracted from human ES cells, T-iPS cells, redifferentiated T cells, peripheral blood T cells, and peripheral blood NK cells using an RNeasy® Micro Kit (manufactured by Qiagen). .. Fluorescently labeled complementary RNAs of each of the total RNAs and Whole Human Genome Microarray 4×44K (G4112F, manufactured by Agilent Technologies) or SurePrint G3 Human Gene Expression 8×60K (G4851A, manufactured by Agilent Technologies) were hybridized in a one-color protocol. .. The resulting signal data were analyzed using a GeneSpring GX software (manufactured by Agilent Technologies).

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.

    other:

    Article Title: Aging-associated Alterations in the Gene Regulatory Network Landscape Associate with Risk, Prognosis and Response to Therapy in Lung Adenocarcinoma
    Article Snippet: This study used Agilent-014850 Whole Human Genome Microarray 4×44K G4112F and Agilent-028004 SurePrint G3 Human GE 8×60K Microarray for sequencing.

    Article Title: Development and evaluation of a chronic kidney disease risk prediction model using random forest
    Article Snippet: The gene expression was analyzed using the Agilent-014850 Whole Human Genome Microarray 4 × 44K G4112F (Probe Name version).

    Article Title: Blocking M2-like macrophage polarization using decoy oligodeoxynucleotide-based gene therapy prevents immune evasion for pancreatic cancer treatment
    Article Snippet: The gene microarray data were analyzed using the Agilent Human whole genome oligo 4*44k chip (array kit serial number: US00082833) and Agilent’s gene expression microarray software, including Feature Extraction and GeneSpring GX.

    Hybridization:

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques
    Article Snippet: .. The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA). .. After hybridization at 65 °C for 17 h, the slides were cleansed using Gene Expression Wash Buffers 1 and 2 (Agilent Technologies) according to the instructions provided by the manufacturer.



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    Points in time for sublingual vaccinations and assessments of anti-HA (hemagglutinin) antibodies, blood tests, plasma cytokines, quantitative reverse transcription PCR (RT-qPCR), and DNA <t>microarray</t> analyses. RT-qPCR and DNA microarray analyses were conducted with RNA isolated from white blood cells (WBC). Vaccinations were performed four times at 0 (1st), 6 (2nd), 18 (3rd), and 30 (4th) weeks. Arrows indicate sampling timepoints for each assay.
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    Image Search Results


    Points in time for sublingual vaccinations and assessments of anti-HA (hemagglutinin) antibodies, blood tests, plasma cytokines, quantitative reverse transcription PCR (RT-qPCR), and DNA microarray analyses. RT-qPCR and DNA microarray analyses were conducted with RNA isolated from white blood cells (WBC). Vaccinations were performed four times at 0 (1st), 6 (2nd), 18 (3rd), and 30 (4th) weeks. Arrows indicate sampling timepoints for each assay.

    Journal: Vaccines

    Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques

    doi: 10.3390/vaccines12060643

    Figure Lengend Snippet: Points in time for sublingual vaccinations and assessments of anti-HA (hemagglutinin) antibodies, blood tests, plasma cytokines, quantitative reverse transcription PCR (RT-qPCR), and DNA microarray analyses. RT-qPCR and DNA microarray analyses were conducted with RNA isolated from white blood cells (WBC). Vaccinations were performed four times at 0 (1st), 6 (2nd), 18 (3rd), and 30 (4th) weeks. Arrows indicate sampling timepoints for each assay.

    Article Snippet: The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Reverse Transcription, Quantitative RT-PCR, Microarray, Isolation, Sampling

    Overview of Methodological Details of Either qRT-PCR (Quantitative Reverse Transcription Polymerase Chain Reaction) or Microarrays Used by the Contributing Teams

    Journal: Radiation research

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay

    doi: 10.1667/RADE-22-00206.1

    Figure Lengend Snippet: Overview of Methodological Details of Either qRT-PCR (Quantitative Reverse Transcription Polymerase Chain Reaction) or Microarrays Used by the Contributing Teams

    Article Snippet: Labeled cRNA samples were applied on the DNA microarray slides (44k whole human genome, G4112F, Agilent).

    Techniques: Reverse Transcription, Polymerase Chain Reaction, Microarray, Isolation, Lysis, Concentration Assay, Sequencing, Labeling, SYBR Green Assay, Multiplex Assay, TaqMan Assay, Real-time Polymerase Chain Reaction, Software, Extraction

    Overview of Methodological Details of Either qRT-PCR (Quantitative Reverse Transcription Polymerase Chain Reaction) or Microarrays Used by the Contributing Teams

    Journal: Radiation research

    Article Title: RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay

    doi: 10.1667/RADE-22-00206.1

    Figure Lengend Snippet: Overview of Methodological Details of Either qRT-PCR (Quantitative Reverse Transcription Polymerase Chain Reaction) or Microarrays Used by the Contributing Teams

    Article Snippet: Additionally, all column RNA prep kits remove most of the DNA. (−) RT control conventional PCR (ß-actin primer, HotStar MasterMix (Qiagen), 30 cycles) Check DNA conta mination No cDNA synthesis cDNA synthesis Kit/MasterMix High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) QuantiTect Reverse Transcription (Qiagen) High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) High Capacity cDNA Archive Kit High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) Kit/MasterMix Quick Amp Labeling Kit (Agilent) PCR protocol 1× /25°C/10min, 1×/37°C/ 120min, 1×/85°C/5min 1×/25°C/10min, 1×/37°C/120min, 1×/85°C/5min 1×/42°C/2min, 1×/42°C/20min, 1×/95°C/3min 1×/25°C/10min, 1×/37°C/120min, 1×/85°C/5min 1×/25°C/5min, 1×/42°C/60min, 1×/l0°C/5min 1×/25°C/10min, 1×/37°C/120min, 1× /85°C/5min 1×/25°C/10min, 1×/37°C/120min PCR protocol 1×/40°C/120min, 1×/70°C/15min; 1 × /40°C/120min Quality control UBC Ct ITFG1 Ct, DPM1 Ct MRPS5 Ct No HPRT1 Ct 18S rRNA Ct Quality control NanoDrop ™ qRT-PCR Kit/MasterMix TaqMan Universal Master Mix TaqMan Universal Master Mix II, no UNG (Thermo Fisher Scientific) QuantiFast SYBR Green PCR (Qiagen) 5X HOT FIREPol ® EvaGreen ® qPCR SuperMix, Solis BioDyne TaqMan fast advanced master mix (Applied Biosystems) and Maxima SYBR Green qPCR Master Mix (Thermo Scientific) TaqMan,PerfeCTa ® , MultiPlex qPCR SuperMix, Quanta bioscience TaqMan Universal Master Mix Microarray DNA-Microarray Agilent, 44k whole human genome, G4112F TaqMan assays SYBR Green assay FDXR (Hs00244586_ml), GDF15 (Hs00171132_ml) BAX (Hs00180269_ml), BBC3 (Hs00248075_ml), CDKN1A (Hs00355782_ml), DDB2 (Hs03044953_ml), FDXR (Hs00244586_ml), GADD45A (Hs00169255_ml), GDF15 (Hs00171132_ml), TNFSF4 (Hs00182411_ml) CDKN1A-F: AGACCAGCATGACAGATTTCTACC; CDKN1A-R: CTTCCTGTGGGCGGATTAGG; DDB2-F: AGCATCACTGGGCTGAAGTT; DDB2-R: TGGTGTCTGAGCTGGCAAAA; FDX-F: TGGAGAGAACGGACATCACG; FDX-R: AGCCACACTGTCTTCACTCG GADD45a for: ACTGCGTGCTGGTGACGAAT, GADD45a rev: GTTGACTTAAGGCAGGATCCTTCCA; FDXR for: TGGATGTGCCAGGCCTCTAC, FDXR rev: TGAGGAAGCTGTCAGTCATGGTT; CDKN1A for: CCTGGAGACTCTCAGGGTCGAAA, CDKN1A rev: GCGTTTGGAGTGGTAGAAATCTGTCA; MDM2 for: TATCAGGCAGGGGAGAGTGATACA, MDM2 rev: CCAACATCTGTTGCAATGTGATGGAA; 18S for: GCTTAATTTGACTCAACACGGGA, 18S rev: AGCTATCAATCTGTCAATCCTGTCC.

    Techniques: Reverse Transcription, Polymerase Chain Reaction, Microarray, Isolation, Lysis, Concentration Assay, Sequencing, Labeling, SYBR Green Assay, Multiplex Assay, TaqMan Assay, Real-time Polymerase Chain Reaction, Software, Extraction